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Julie Kiefer
Director, Research Communications, 91麻豆天美直播
Email: Julie.Kiefer@hsc.utah.edu
The pandemic and its aftermath have raised anxiety to new levels. But the roots of anxiety-related conditions, including obsessive-compulsive spectrum disorder (OCSD), are still unclear. In a new study, 91麻豆天美直播 scientists discovered insights into the importance of a minor cell type in the brain鈥攎icroglia鈥攊n controlling anxiety-related behaviors in laboratory mice. Traditionally, neurons鈥攖he predominant brain cell type鈥攁re thought to control behavior.
The researchers showed that, like buttons on a game controller, specific microglia populations activate anxiety and OCSD behaviors while others dampen them. Further, microglia communicate with neurons to invoke the behaviors. The findings, published in , could eventually lead to new approaches for targeted therapies.
鈥淎 small amount of anxiety is good,鈥 says ., a distinguished professor of human genetics at the Spencer Fox Eccles School of Medicine at University of Utah and senior author of the study. 鈥淎nxiety motivates us, spurs us on, and gives us that extra bit of push that says, 鈥業 can.鈥 But a large dose of anxiety overwhelms us. We become mentally paralyzed, the heart beats faster, we sweat, and confusion settles in our minds.鈥
鈥淭his work is unique and has challenged the current dogma about the role of microglia function in the brain.鈥
The newly identified mechanisms could be important for maintaining behaviors within the healthy range under normal conditions. Under pathological conditions, the mechanisms could drive behaviors that become debilitating, Capecchi says.
鈥淭his work is unique and has challenged the current dogma about the role of microglia function in the brain,鈥 says Naveen Nagarajan, Ph.D, a geneticist and neuroscientist at U of U Health and the study鈥檚 lead author.
Manipulating microglia
Mice with OCSD-like behaviors can鈥檛 resist grooming themselves. They lick their bodies so much that their fur sloughs off, and they develop welts. Previously, Capecchi鈥檚 team discovered that a mutation in a gene called Hoxb8 caused mice to show signs of chronic anxiety and to groom themselves excessively. Unexpectedly, they identified that the source of these behaviors was a type of immune cell called microglia. Accounting for only 10% of cells in the brain, microglia had been thought of as the brain鈥檚 鈥渢rash collectors鈥 that disposed of dying neurons鈥攖he most common brain cell鈥攁nd abnormally shaped proteins. Their discoveries were also among the first to reveal that Hoxb8 microglia were important for controlling behavior by communicating with specific neuronal circuits.
But how microglia accomplished these tasks remained a mystery. To learn more, Nagarajan turned to optogenetics, a technique that combines laser light and genetic engineering. Like playing a video game, he used the laser to stimulate specific populations of microglia in the brain.
To the researchers鈥 amazement, they could turn on anxiety-related behaviors with the flip of a switch. When they used the laser to stimulate one subpopulation, Hoxb8 microglia, the mice became more anxious. When the laser triggered Hoxb8 microglia in other parts of the brain, the mice groomed themselves. Targeting Hoxb8 microglia in yet another location had multiple effects: the mice鈥檚 anxiety increased, they groomed themselves, and they froze, an indicator of fear. Whenever the scientists turned the laser off, the behaviors stopped.
鈥淭hat was a big surprise for us,鈥 Nagarajan says. 鈥It is conventionally thought that only neurons can generate behaviors. The current findings shed light on a second way that the brain generates behaviors using microglia.鈥 In fact, stimulating microglia with the laser caused the neurons sitting next to them to fire more strongly, suggesting that the two cell types communicate with one another to drive distinct behaviors.
Further experiments revealed yet another layer of control by a population of microglia that do not express Hoxb8. Stimulating 鈥渘on-Hoxb8鈥 and Hoxb8 microglia at the same time prevented the onset of anxiety and OCSD-like behaviors. These results suggested that the two populations of microglia act like a brake and an accelerator. They balance each other out under normal conditions and induce a disease state when the signals are off-balance.
The research shows that location and type of microglia are two characteristics that appear to be important for fine-tuning anxiety and OCSD behaviors. From there, microglia communicate with specific neurons and neural circuits that ultimately control behavior, Capecchi says. 鈥淲e want to learn more about the two-way communications between neurons and microglia,鈥 he says. 鈥淲e want to know what鈥檚 responsible for that.鈥 Defining these interactions in mice could lead to therapeutic targets for controlling excessive anxiety in patients.
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The study, 鈥淥ptogenetic stimulation of mouse Hoxb8 in specific regions of the brain induces anxiety, grooming, or both,鈥 appears in